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Upright microscope for brightfield and epifluorescence microscopy

The Bioimaging Hub operates a number of conventional widefield research microscopes that offer a variety of optical contrast techniques. All are equipped with modern networked PC workstations and digital image capture.

The Leica DMLB upright microscope is suitable for colour brightfield and multichannel (RGB) epifluorescence imaging of histological tissue sections.

Make/model Leica DMLB epifluorescence and brightfield photomicroscope
Details Suitable for histological analysis and multichannel (RGB) epifluorescence of slide-mounted tissue samples.
Facility Bioimaging Research Hub
School School of Biosciences

The Leica DMLB microscope has 2.5x, 5x, 10x, 20x, 40x and 63x objectives and RGB reflector cubes for epifluorescence. The microscope is equipped with a Jenoptik Progres CFscan colour camera and Progres Capture Pro software.

Objectives

  • Leica 2.5x/0.07 PL Fluotar
  • Leica 5x/0.12 PL Fluotar
  • Leica 10x/0.30 HC PL Fluotar
  • Leica 20x/0.50 HC PL Fluotar
  • Leica 40x/0.70 PL Fluotar
  • Leica 63x/0.70 (corr) HCX PL Fluotar

Internal users can book the equipment using the shared ‘BIOSI - Leica DMLB' Outlook calendar.

Find out how to book through Outlook.

The booking will be reviewed and then accepted by a member of Bioimaging Unit staff or an alternative time/date proposed.

Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.

Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will still be charged.

Please contact bioimaginghub@cardiff.ac.uk for details of costs.

All users must be trained and registered by Bioimaging Hub staff before using any of the instruments within the facility.

To comply with Cardiff University Health and Safety Policy, individuals must receive appropriate training before using any of the imaging equipment within the Bioimaging Unit and be aware of the risks involved in its use.

If top-up training is required, please notify Unit staff when making a booking and this can be provided prior to your planned session.

All users of the Bioimaging Unit are expected to observe the following rules and regulations. Failure to comply will result in loss of access rights.

  • Food and drink are not permitted within the facility.
  • All waste material (including laboratory gloves) should be properly disposed of outside of the facility – we are not responsible for your waste.
  • Coats and bags when left on the floor in a low light environment represent a tripping hazard. Either hang them on the internal door hooks or, better still, do not bring them into the imaging suites.
  • Do not use any equipment that you have not been properly trained for.  All users should familiarise themselves with the appropriate risk assessments for the procedures being undertaken.
  • All USB data storage devices must be virus checked before plugging them into any of our systems.
  • All systems should be correctly powered down at the end of your session and usage correctly logged in the database.
  • Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.
  • Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.

Get in touch

Dr Anthony J Hayes

Email
hayesaj@cardiff.ac.uk
Telephone
+44 (0)29 2087 6611

Location

E/0.04
Sir Martin Evans Building
Museum Avenue
CF10 3AX

Opening hours

The Unit is open Monday-Friday 9am-5pm. If you require access outside these hours please contact the facility administrator.