Upright, fully-automated, multimodal research microscope
The Bioimaging Unit operates a number of widefield research microscopes housed within a recently refurbished microscopy suite.
These systems offer a variety of optical contrast techniques and are all equipped with digital image capture.
The Olympus BX61 is a fully-automated upright microscope that is suitable for brightfield, differential interference contrast and epifluorescence imaging.
This system is available with or without technical support.
| Make/model | Olympus BX61 microscope with Hamamatsu ORCA Spark digital camera |
|---|---|
| Details | Suitable for brightfield, differential interference contrast and epifluorescence imaging. The system is fully motorised and has a stepping stage that allows acquisition of 3D image data series. |
| Facility | Bioimaging Research Hub |
| School | School of Biosciences |
The instrument has x4, x10, x20, x40 and x100 (oil) lenses and RGB filters for epifluorescence. It is equipped with a Hamamatsu ORCA Spark B&W digital camera and cellSens Dimension advanced imaging software. The system is fully motorised and has a stepping stage that allows acquisition of 3D image data series.
Objectives
- 4x/0.13 UPlan Fl
- 10x/0.30 UPlan Fl
- 20x/0.50 UPlan Fl
- 40x/0.75 UPlan Fl
- 100x/1.35 UPlanApo Oil (iris)
Internal users can book the equipment using the shared Outlook calendar - 'BIOSI - E/0.04 - widefield microscopy'.
Details of how to book equipment through Outlook.
The booking will be reviewed and then accepted by a member of Bioimaging Hub staff or an alternative time/date proposed.
Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.
Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.
Please contact Bioimaging Hub for details of costs - Bioimaginghub@cardiff.ac.uk
All users must be trained and registered by Bioimaging Unit staff before using any of the instruments within the Unit.
User guides
To comply with Cardiff University Health and Safety Policy, individuals must receive appropriate training before using any of the imaging equipment within the Bioimaging Unit and be aware of the risks involved in its use.
If top-up training is required, please notify Unit staff when making a booking and this can be provided prior to your planned session.
All users of the Bioimaging Unit are expected to observe the following. If they fail to comply they will lose their access rights:
- Food and drink are not permitted within the facility.
- All waste material (including laboratory gloves) should be properly disposed of outside of the facility – we are not responsible for your waste.
- Coats and bags when left on the floor in a low light environment represent a tripping hazard. Either hang them on the internal door hooks or, better still, do not bring them into the imaging suites.
- Do not use any equipment that you have not been properly trained for. All users should familiarise themselves with the appropriate risk assessments for the procedures being undertaken.
- All USB data storage devices must be virus checked before plugging them into any of our systems.
- All systems should be correctly powered down at the end of your session and usage correctly logged in the database.
- Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.
- Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.
Get in touch
Dr Anthony J Hayes
- hayesaj@cardiff.ac.uk
- +44 (0)29 2087 6611
Location
Sir Martin Evans Building
Museum Avenue
CF10 3AX