Inverted epifluorescence microscope for multiformat imaging
The Bioimaging Hub operates a number of conventional widefield research microscopes that offer a wide variety of optical contrast techniques. All are equipped with modern networked PC workstations and digital image capture.
The Olympus IX71 inverted epifluorescence microscope has a Hamamatsu Orca Spark CMOS monochrome camera and Olympus cellSens Standard edition software. A small incubator is available within the Hub for short term culture of cells.
| Make/model | Olympus IX71 inverted microscope with Hamamatsu ORCA Spark digital camera |
|---|---|
| Details | Suitable for epifluorescence (RGB) and phase contrast examination of live or fixed cells and tissue samples on slides, petri-dishes, and multi-well plates. |
| Facility | Bioimaging Research Hub |
| School | School of Biosciences |
The Olympus IX71 inverted microscope has 2x, 5x, 10x, 20x and 40x objectives and RGB reflector cubes for epifluorescence. The microscope is equipped with a Hamamatsu ORCA Spark monochrome CMOS camera and Olympus cellSens Standard edition imaging software.
Objectives
- 2x/0.05 Plan
- 5x/0.15 UMPlan Fl
- 10x/0.30 Ph1 UPlan Fl
- 20x/0.40 Ph1 LCPlan Fl
- 40x/0.60 Ph2 LCPlan Fl
Internal users can book the equipment using the shared 'BIOSI - Olympus IX71' Outlook calendar.
Find out how to book through Outlook.
The booking will be reviewed and then accepted by a member of Bioimaging Hub staff or an alternative time/date proposed.
Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.
Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.
Please contact Bioimaging Hub for details of costs:
All users must be trained and registered by Bioimaging Unit staff before using any of the instruments within the Unit.
To comply with Cardiff University Health and Safety Policy, individuals must receive appropriate training before using any of the imaging equipment within the Bioimaging Unit and be aware of the risks involved in its use.
If top-up training is required, please notify Unit staff when making a booking and this can be provided prior to your planned session.
All users of the Bioimaging Unit are expected to observe the following rules and regulations. Failure to comply will result in loss of access rights:
- Food and drink are not permitted within the facility.
- All waste material (including laboratory gloves) should be properly disposed of outside of the facility – we are not responsible for your waste.
- Coats and bags when left on the floor in a low light environment represent a tripping hazard. Either hang them on the internal door hooks or, better still, do not bring them into the imaging suites.
- Do not use any equipment that you have not been properly trained for. All users should familiarise themselves with the appropriate risk assessments for the procedures being undertaken.
- All USB data storage devices must be virus checked before plugging them into any of our systems.
- All systems should be correctly powered down at the end of your session and usage correctly logged in the database.
- Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.
- Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.
Get in touch
Dr Anthony J Hayes
- hayesaj@cardiff.ac.uk
- +44 (0)29 2087 6611
Location
Sir Martin Evans Building
Museum Avenue
CF10 3AX