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Smart diagnostics

Synthetic materials inspired by biological cells could provide the next generation of smart diagnostics.

Dr Castell and his team have developed new materials using water and oil which could play a vital role in healthcare and diagnostics in the future. Using the science of microfluidics, the technique manipulates tiny volumes of fluid to create a series of interconnected water droplets inside a small droplet of oil, itself encased within a gel-like, semi-permeable shell.

Detailed in the Journal Angewandte Chemie (Open Access) these new materials are inspired by biological cells and their development makes it possible to take previously fragile molecular membranes outside of the laboratory and interface them with the wider world.

Moving membrane based droplet networks out of the lab

Membrane based droplet networks, able to incorporate protein machinery and display emergent functional properties, have been predicted to find vital roles in future healthcare and diagnostic applications. However, they have so far typically not fared well outside well controlled laboratory conditions.

By using microfluidics to surround these systems with a thin hydrogel shell, the team have been able to significantly stabilise the membrane networks, providing mechanical rigidity whilst maintaining environmental interaction, facilitating their use in environments outside of the laboratory.

The development of these materials underpins a €4.4M EU research project - Artificial Cells with Distributed Cores to Decipher Protein Function - that seeks inspiration from biology to recapitulate some aspects of biological functionality arising from chemical compartmentalisation.

It is envisaged that such artificial cell technology will ultimately be used as programmable and reconfigurable matter for a range of applications from smart diagnostics to drug delivery, to chemical synthesis and energy harvesting.

bilayer droplets
1. A multi-core protocell made up of membrane separated water droplets in an oil environment surrounded by a gel capsule sits stably, outside the normal lab environment, on a leaf 2. The membrane separated compartments of a microfluidically produced protocell catch the light as they sit stably on a twig able to interface with the natural environment. 3. The birth of a protocell: Freshly made membrane compartmentalised eDIBs (encapsulated droplet interface bilayers) rest in their microfluidic housing before being released into the lab for testing. 4. A single membrane compartmentalised protocell sits on a microscope slide where the chemistries of its internal cores can be observed by researchers.

Paper

Baxani D. et al. ‘Bilayer Networks within a Hydrogel Shell: A Robust Chassis for Artificial Cells and a Platform for Membrane Studies' Angewandte Chemie International Edition, Volume 55, Issue 46, November 7, 2016 Pages 14240–14245. (Open Access).

More information

For more information about this project, please contact:

Dr Oliver Castell

Dr Oliver Castell

Serious Brain Power Early Career Researcher and Senior Lecturer

Email
castello@cardiff.ac.uk
Telephone
+44 (0)29 2087 6241