Spinning Disc Confocal Microscope
A live cell imaging spoke of the Bioimaging Research Hub houses a spinning disc system for fast, time-lapse confocal imaging applications. The microscope enables high speed, multi-position (x,y,z), multi-colour fluorescence image acquisition. Support systems for live cell imaging (i.e. gas and incubation) are also available within the facility.
| Make/model | Olympus IX71 motorised, inverted microscope |
|---|---|
| Details | Crest Optics X-Light V2 confocal imager; Cairn Research tri-line laser bank (405nm; 488nm; 561nm); Hamamatsu ORCA Flash 4 CMOS digital camera with M-View Gemini image splitter |
| Facility | Bioimaging Research Hub |
| School | School of Biosciences |
The instrument has x4, x10, x20, x40, x60(oil) and x100 (oil) objective lenses and RGB filters for epifluorescence. It has a PriorScan II motorised (x,y,z) stage, a motorised filter turret and shutter with filters for visualisation of DAPI, FITC, TRITC and CFP, YFP and RFP. It is equipped with a Crest Optics X-Light V2 spinning disc confocal scan head, a tri-line laser bank (405nm, 488nm, 561nm) and a Hamamatsu ORCA 4.0 Flash 4.0 CMOS monochrome digital camera, The microscope system is controlled through Molecular Devices MetaMorph microscopy automation and image analysis software.
Objectives
- 4x/0.13 UPlan FLN
- 10x/0.30 UPlan Fl
- 20x/0.45 LUCPlan FLN
- 40x/0.60 LUCPlan FLN
- 60x/1.42 PlanApo N Oil
- 100x/1.40 UPlanSApo Oil
Lasers
- Diode 405nm (100mW)
- Diode 488nm (150mW)
- Diode 561nm (100mW)
Internal users can book the equipment using the shared Outlook calendar ‘BIOSI - Olympus IX71 live cell system’.
Find out how to book equipment through Outlook.
The booking will be reviewed and then accepted by a member of Bioimaging Unit staff or an alternative time/date proposed.
Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is one hour.
Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.
For costs, please contact the Academic Lead for Imaging:
Professor Pete Watson
Email: watsonpd@cardiff.ac.uk
Phone: +44 (0)29 2087 9042
For training, please contact the Academic Lead for Imaging:
Professor Pete Watson
Email: watsonpd@cardiff.ac.uk
Phone: +44 (0)29 2087 9042
To comply with Cardiff University Health and Safety Policy, individuals must receive appropriate training before using the system and be aware of the risks involved in its use.
Users must:
- Receive training from an approved member of staff (indirect training from other users will not be sufficient) - refer to Training and Support section.
- Read and understand the latest Risk Assessment from the Bioimaging Hub.
- Complete a Registration of Laser Worker form.
All users are expected to observe the following rules and regulation. Failure to comply will result in loss of access rights:
- Food and drink are not permitted within the facility.
- All waste material (including laboratory gloves) should be properly disposed of outside of the facility.
- Coats and bags when left on the floor in a low light environment represent a tripping hazard. Either hang them on the internal door hooks or, better still, do not bring them into the imaging suites.
- Do not use any equipment that you have not been properly trained for. All users should familiarise themselves with the appropriate risk assessments for the procedures being undertaken.
- All USB data storage devices must be virus checked before plugging them into any of our systems.
- All systems should be correctly powered down at the end of your session and usage correctly logged in the database.
Get in touch
Dr Peter Watson
- watsonpd@cardiff.ac.uk
- +44 (0)29 2087 9042
Location
Sir Martin Evans Building
Museum Avenue
CF10 3AX